Andrew Tate and his brother Tristan appealed with Bucharest's Court of Appeal to be let out.
They were denied by a Romanian judge who voiced concerns about their escaping to a country against extradition.
The brothers' lawyers presented many arguments, including the fact that they had children.
Instead, the judge clamped down harder by upholding their 30-day detention.
They were arrested on charges of human trafficking, organized crime, and rape alongside two women.
Andrew was seen holding a Qur'an as he walked into the court handcuffed to his brother.
The display caused a reaction on Twitter, with some in support while others opposed it.
Greta Thunberg posted a petty tweet responding to the misogynistic influencer's arrest.
This prompted an onslaught of hilarious responses from other Twitter users.
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